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vector expressing gfp cyclin b1  (Addgene inc)


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    Addgene inc vector expressing gfp cyclin b1
    The rs11556924 SNP alters <t>cyclin-B1</t> dynamics. A , level of Cyclin-B1 in isogenic cell lines of different genotypes was examined using quantitation of Western blots. Example blot shown in ( i ), quantitation relative to β-actin expression shown in ( ii ) ( p = 0.017). N numbers represent individual cell lines, reactions were carried out in technical triplicates, and data combined from two independent experiments. B , to examine the rate of cyclin-B1 nuclear accumulation, <t>Cyclin-B1-GFP</t> was expressed in CAD-risk and CAD-non-risk cells and its live cell imaging used to monitor its accumulation at 2-min intervals. ( i ) In these examples, in the risk cells, nuclear import begins at −6 min, in the risk example, nuclear accumulation begins at −4 min (0 min is the time at which the nuclear/cytoplasmic ratio rises above 1). ( ii ) Boxplot showing overall data for CAD-risk and CAD-non-risk genotype cells ( p = 0.004). C , to further examine the stability of Cyclin-B1 in our isogenic cell lines, we blocked protein synthesis with cycloheximide, and then examined the level of Cylin-B1 after 5 h of treatment. Example blot shown in ( i ) from these data, the relative amount of Cyclin-B1 remaining after 5 h shown in ( ii ) ( p = 0.167). Data were combined from four experiments of 2 cell lines of each genotype. Error bars indicate standard deviation.
    Vector Expressing Gfp Cyclin B1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+expressing+gfp+cyclin+b1/pCMX%2FcyclinB1-GFP+(27)+(Plasmid+%2326061)/pmc04965579-177-8-20
    Average 93 stars, based on 7 article reviews
    vector expressing gfp cyclin b1 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "The Coronary Artery Disease-associated Coding Variant in Zinc Finger C3HC-type Containing 1 (ZC3HC1) Affects Cell Cycle Regulation * "

    Article Title: The Coronary Artery Disease-associated Coding Variant in Zinc Finger C3HC-type Containing 1 (ZC3HC1) Affects Cell Cycle Regulation *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.734020

    The rs11556924 SNP alters cyclin-B1 dynamics. A , level of Cyclin-B1 in isogenic cell lines of different genotypes was examined using quantitation of Western blots. Example blot shown in ( i ), quantitation relative to β-actin expression shown in ( ii ) ( p = 0.017). N numbers represent individual cell lines, reactions were carried out in technical triplicates, and data combined from two independent experiments. B , to examine the rate of cyclin-B1 nuclear accumulation, Cyclin-B1-GFP was expressed in CAD-risk and CAD-non-risk cells and its live cell imaging used to monitor its accumulation at 2-min intervals. ( i ) In these examples, in the risk cells, nuclear import begins at −6 min, in the risk example, nuclear accumulation begins at −4 min (0 min is the time at which the nuclear/cytoplasmic ratio rises above 1). ( ii ) Boxplot showing overall data for CAD-risk and CAD-non-risk genotype cells ( p = 0.004). C , to further examine the stability of Cyclin-B1 in our isogenic cell lines, we blocked protein synthesis with cycloheximide, and then examined the level of Cylin-B1 after 5 h of treatment. Example blot shown in ( i ) from these data, the relative amount of Cyclin-B1 remaining after 5 h shown in ( ii ) ( p = 0.167). Data were combined from four experiments of 2 cell lines of each genotype. Error bars indicate standard deviation.
    Figure Legend Snippet: The rs11556924 SNP alters cyclin-B1 dynamics. A , level of Cyclin-B1 in isogenic cell lines of different genotypes was examined using quantitation of Western blots. Example blot shown in ( i ), quantitation relative to β-actin expression shown in ( ii ) ( p = 0.017). N numbers represent individual cell lines, reactions were carried out in technical triplicates, and data combined from two independent experiments. B , to examine the rate of cyclin-B1 nuclear accumulation, Cyclin-B1-GFP was expressed in CAD-risk and CAD-non-risk cells and its live cell imaging used to monitor its accumulation at 2-min intervals. ( i ) In these examples, in the risk cells, nuclear import begins at −6 min, in the risk example, nuclear accumulation begins at −4 min (0 min is the time at which the nuclear/cytoplasmic ratio rises above 1). ( ii ) Boxplot showing overall data for CAD-risk and CAD-non-risk genotype cells ( p = 0.004). C , to further examine the stability of Cyclin-B1 in our isogenic cell lines, we blocked protein synthesis with cycloheximide, and then examined the level of Cylin-B1 after 5 h of treatment. Example blot shown in ( i ) from these data, the relative amount of Cyclin-B1 remaining after 5 h shown in ( ii ) ( p = 0.167). Data were combined from four experiments of 2 cell lines of each genotype. Error bars indicate standard deviation.

    Techniques Used: Quantitation Assay, Western Blot, Expressing, Live Cell Imaging, Standard Deviation



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    vector expressing gfp cyclin b1  (Addgene inc)
    93
    Addgene inc vector expressing gfp cyclin b1
    The rs11556924 SNP alters <t>cyclin-B1</t> dynamics. A , level of Cyclin-B1 in isogenic cell lines of different genotypes was examined using quantitation of Western blots. Example blot shown in ( i ), quantitation relative to β-actin expression shown in ( ii ) ( p = 0.017). N numbers represent individual cell lines, reactions were carried out in technical triplicates, and data combined from two independent experiments. B , to examine the rate of cyclin-B1 nuclear accumulation, <t>Cyclin-B1-GFP</t> was expressed in CAD-risk and CAD-non-risk cells and its live cell imaging used to monitor its accumulation at 2-min intervals. ( i ) In these examples, in the risk cells, nuclear import begins at −6 min, in the risk example, nuclear accumulation begins at −4 min (0 min is the time at which the nuclear/cytoplasmic ratio rises above 1). ( ii ) Boxplot showing overall data for CAD-risk and CAD-non-risk genotype cells ( p = 0.004). C , to further examine the stability of Cyclin-B1 in our isogenic cell lines, we blocked protein synthesis with cycloheximide, and then examined the level of Cylin-B1 after 5 h of treatment. Example blot shown in ( i ) from these data, the relative amount of Cyclin-B1 remaining after 5 h shown in ( ii ) ( p = 0.167). Data were combined from four experiments of 2 cell lines of each genotype. Error bars indicate standard deviation.
    Vector Expressing Gfp Cyclin B1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+expressing+gfp+cyclin+b1/pCMX%2FcyclinB1-GFP+(27)+(Plasmid+%2326061)/pmc04965579-177-8-20
    Average 93 stars, based on 1 article reviews
    vector expressing gfp cyclin b1 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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    The rs11556924 SNP alters cyclin-B1 dynamics. A , level of Cyclin-B1 in isogenic cell lines of different genotypes was examined using quantitation of Western blots. Example blot shown in ( i ), quantitation relative to β-actin expression shown in ( ii ) ( p = 0.017). N numbers represent individual cell lines, reactions were carried out in technical triplicates, and data combined from two independent experiments. B , to examine the rate of cyclin-B1 nuclear accumulation, Cyclin-B1-GFP was expressed in CAD-risk and CAD-non-risk cells and its live cell imaging used to monitor its accumulation at 2-min intervals. ( i ) In these examples, in the risk cells, nuclear import begins at −6 min, in the risk example, nuclear accumulation begins at −4 min (0 min is the time at which the nuclear/cytoplasmic ratio rises above 1). ( ii ) Boxplot showing overall data for CAD-risk and CAD-non-risk genotype cells ( p = 0.004). C , to further examine the stability of Cyclin-B1 in our isogenic cell lines, we blocked protein synthesis with cycloheximide, and then examined the level of Cylin-B1 after 5 h of treatment. Example blot shown in ( i ) from these data, the relative amount of Cyclin-B1 remaining after 5 h shown in ( ii ) ( p = 0.167). Data were combined from four experiments of 2 cell lines of each genotype. Error bars indicate standard deviation.

    Journal: The Journal of Biological Chemistry

    Article Title: The Coronary Artery Disease-associated Coding Variant in Zinc Finger C3HC-type Containing 1 (ZC3HC1) Affects Cell Cycle Regulation *

    doi: 10.1074/jbc.M116.734020

    Figure Lengend Snippet: The rs11556924 SNP alters cyclin-B1 dynamics. A , level of Cyclin-B1 in isogenic cell lines of different genotypes was examined using quantitation of Western blots. Example blot shown in ( i ), quantitation relative to β-actin expression shown in ( ii ) ( p = 0.017). N numbers represent individual cell lines, reactions were carried out in technical triplicates, and data combined from two independent experiments. B , to examine the rate of cyclin-B1 nuclear accumulation, Cyclin-B1-GFP was expressed in CAD-risk and CAD-non-risk cells and its live cell imaging used to monitor its accumulation at 2-min intervals. ( i ) In these examples, in the risk cells, nuclear import begins at −6 min, in the risk example, nuclear accumulation begins at −4 min (0 min is the time at which the nuclear/cytoplasmic ratio rises above 1). ( ii ) Boxplot showing overall data for CAD-risk and CAD-non-risk genotype cells ( p = 0.004). C , to further examine the stability of Cyclin-B1 in our isogenic cell lines, we blocked protein synthesis with cycloheximide, and then examined the level of Cylin-B1 after 5 h of treatment. Example blot shown in ( i ) from these data, the relative amount of Cyclin-B1 remaining after 5 h shown in ( ii ) ( p = 0.167). Data were combined from four experiments of 2 cell lines of each genotype. Error bars indicate standard deviation.

    Article Snippet: For Cyclin-B1-GFP imaging, cells were transfected with a vector expressing GFP-Cyclin-B1 (pCMX/cyclinB1-GFP ( ) was a gift from Jonathon Pines (Addgene plasmid 26061)) using Lipofectamine LTX (Fisher Scientific).

    Techniques: Quantitation Assay, Western Blot, Expressing, Live Cell Imaging, Standard Deviation